Insertion junction: CLIP2.053527_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.053527
Chromosome:chromosome 17
Location:3053897
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g720150 (1 of 1) IPR000104//IPR008752 - Antifreeze protein, type I // Peptidase M11, gametolysin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTCGGCCCATCCGAACACGTCGGCGACGGAGCACCGCGCCGCGCTCCA
Internal bar code:GAAAAAATGTTCCTGGGGGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2222
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAACCTTGCGCTTCAGGAAC
Suggested primer 2:AGTCGCAATCGTCACAGGAG