Insertion junction: CLIP2.053531_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.053531
Chromosome:chromosome 10
Location:1176274
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g425800 ORN1 (1 of 1) 3.1.13.3 - Oligonucleotidase; Putative organellar oligoribonuclease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCGCGTGAGGGCGGTGCTGACGGTAGCCGCCTTCGAAATTGCCACCC
Internal bar code:GGGTTTGCTGTTAGGCACTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:620
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTACATCCAGACGTCTCCGC
Suggested primer 2:AGCCGGATTCTCTAGCTTGC