Insertion junction: CLIP2.053548_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.053548
Chromosome:chromosome 1
Location:4289488
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g029050 (1 of 1) PF08513//PF10607 - LisH (LisH) // CTLH/CRA C-terminal to LisH motif domain (CLTH) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCCGTGTCGCCGCCGCGGATGAGCTCTATGAGCCGCTGCTGCTGCAG
Internal bar code:GCCGTACCGTTGAACTCGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2334
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGGGGAGCGGGTAACATT
Suggested primer 2:CCACTCAAATCCCTGTCCCC