| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053550 |
| Chromosome: | chromosome 3 |
| Location: | 7357263 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206500 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGCGGCCCCGCATGCCACCGCCGCCCACTGCGCCCGCCCGTGAGCTC |
| Internal bar code: | ACTGCCTGCCGTAGGGTTAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1558 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTAATGGGGGAGCCGTGA |
| Suggested primer 2: | GTAGGTTGGAAGCCCCTGTC |