| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053551 |
| Chromosome: | chromosome 3 |
| Location: | 8618329 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210401 | (1 of 1) PTHR14614//PTHR14614:SF15 - HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGAATTAGGCGTGCTGGGTAAACGTTGTTTAGCGCGCCCAGGGTGCGG |
| Internal bar code: | CTCGCCCGTTTGGCAATAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3225 |
| LEAP-Seq percent confirming: | 78.4615 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 130 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAAGAGGCGGATGGAAA |
| Suggested primer 2: | GGACTGTGTTGCTGACCGTA |