Insertion junction: CLIP2.053556_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.053556
Chromosome:chromosome 1
Location:1789393
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g010000 MAPK8 Mitogen-Activated Protein Kinase; (1 of 1) K14512 - mitogen-activated protein kinase 6 (MPK6) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCATCTCACGCACGGACCTGCCACCCCCTGCATTCCATACCGTCCCTA
Internal bar code:CTAGTTAGCTGTGTTCTTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:4030
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:AGTACTGGCTGTGGTCGTTG
Suggested primer 2:GCACAACACAGAAACCCGAC