Insertion junction: CLIP2.053564_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.053564
Chromosome:chromosome 17
Location:1821591
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g709300 (1 of 1) PF10009 - Uncharacterized protein conserved in bacteria (DUF2252) (DUF2252) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTAACCTTGGGGGGTTCGACTCGCCAGCTGCAGAACGAGAAGACTCTC
Internal bar code:GGCGATGGACTAGATTATTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:997
LEAP-Seq percent confirming:75.6757
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:TGATGATGGACTGGTTGGCC
Suggested primer 2:CATGTGCAGATGGAGCATGC