| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.053569 |
| Chromosome: | chromosome 1 |
| Location: | 3489486 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022750 | FAP159 | Flagellar Associated Protein 159; (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGGAACGCTGCAGCGGCAGAGGGAGGTGATGTGGCTGCGGCGGGCGA |
| Internal bar code: | TTCTCCGCTGATTGGGTACCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3226 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCCTGCAAAAGTTCACAG |
| Suggested primer 2: | AGAGCGCCATCGAGGTAATG |