Insertion junction: CLIP2.053580_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.053580
Chromosome:chromosome 11
Location:2283317
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g801291 (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCAGCTTGGTGGCGGCGGCGGCGGAGGCAATGGGGTTGGTGGCCGAGG
Internal bar code:ACTGTTTTGTTTTGTTTCTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1389
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCGTAGAAGGCAGCCCTA
Suggested primer 2:CGTAGTCGGCGTACTGGAAA