Insertion junction: CLIP2.053587_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.053587
Chromosome:chromosome 11
Location:2281881
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095143 (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGGACAGCAGGGACAGCAGGGACAGCAGGGACAGCAGGGACAGCAGG
Internal bar code:TTATATAGTTGCTTTCAATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:699
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTTCAACGCAACCTCAA
Suggested primer 2:CTCGCACACATACGCACATG