| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053604 |
| Chromosome: | chromosome 2 |
| Location: | 6313541 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387500 | OPR38 | OctotricoPeptide Repeat protein 38 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGACTCCGCGGCCGGCAGGAAGCCCTCCAGCCAGGCAGCCGGCGGCGG |
| Internal bar code: | GGCGTGCATCCCTAGATGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5046 |
| LEAP-Seq percent confirming: | 76.5957 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 141 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTCAAGTACCAGCCCAC |
| Suggested primer 2: | GCCAACAAAACGCCTATGCA |