| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053607 |
| Chromosome: | chromosome 10 |
| Location: | 1322191 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427453 | CDS | ||
| lncRNA_TCONS_00060962 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCGAAACAGGGTGCAGGTTAGCTGGGTGAGCGGCGTGGCACTTCAGG |
| Internal bar code: | ATCTATCTCATTTGATTTAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4978 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGCTATACATTGAGGGCT |
| Suggested primer 2: | CGCCAGAAGCTGTAGGTGAA |