Insertion junction: CLIP2.053619_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.053619
Chromosome:chromosome 6
Location:8457969
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g308650 (1 of 1) IPR003072//IPR003980//IPR005404 - Orphan nuclear receptor, NOR1 type // Histamine H3 receptor // Potassium channel, voltage dependent, Kv3.3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAACCCAACAACTCTATGCTCTCCCCATCTTCCGACACACCCTCCCCC
Internal bar code:ATCCAACAGGGTGATATAGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:203
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAAGTCGCAACCCATATG
Suggested primer 2:CCCTGCCATCGTCATCACTT