Insertion junction: CLIP2.053631_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.053631
Chromosome:chromosome 9
Location:3874007
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGTCAAAATACATGCGTTTCGAATCTATCGAGACCAGGTTTCGATCC
Internal bar code:TCAGATTTAGAGTTGCAGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1378
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACGGTTTCTTGGCCCTG
Suggested primer 2:CGCACACACACTCACACTTG