| Insertion cassette: | CIB2 | 
| Side of cassette: | 5' | 
| Strand: | + | 
| Strain: | CLIP2.053633 | 
| Chromosome: | chromosome 12 | 
| Location: | 3673631 | 
| Confidence (%): | 80 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre12.g513650 | FAP342 | Flagellar Associated Protein 342 | intron | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCGACCGCGCGCCGCCACCCCTCCTGAAGCGATTATGGCCCGTCTGT | 
| Internal bar code: | CGCCACGTTGCGGGATTATAGG | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2866 | 
| LEAP-Seq percent confirming: | 100.0 | 
| LEAP-Seq n confirming: | 102 | 
| LEAP-Seq n nonconfirming: | 0 | 
| LEAP-Seq n unique pos: | 102 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGGAGTCTGTCGCAGT | 
| Suggested primer 2: | CCTGCTCCAATACCCTGCAA |