| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053659 |
| Chromosome: | chromosome 13 |
| Location: | 1448491 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572150 | NAT29 | N-acetyltransferase; (1 of 1) PTHR14744:SF15 - N-ALPHA-ACETYLTRANSFERASE 60 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCACCATCAGCAACATCAGTACCAGGCTTTCCAGCAGCACCAGCAGCA |
| Internal bar code: | GCACAGATACTACAAGGATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2183 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGGTGTTGTGTACGGC |
| Suggested primer 2: | CTCAGCTCGGATGCATAGCA |