| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053663 |
| Chromosome: | chromosome 3 |
| Location: | 5091369 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180350 | NSG16,CKS1 | Cyclin dependent kinase regulatory subunit; (1 of 1) K02219 - cyclin-dependent kinase regulatory subunit CKS1 (CKS1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGTCGCTTGCGCCCAGGCACGTCGTCCTGCCCCCGGAGATTGCCCAG |
| Internal bar code: | TGAGAGCGGTGAAATTTATAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5383 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCCTTGCAGAGCCATTTA |
| Suggested primer 2: | CAAGGAAGGCGCCAAAGTTC |