Insertion junction: CLIP2.053674_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.053674
Chromosome:chromosome 11
Location:1848681
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre11.g467350 ACO2 Acyl-CoA oxidase/dehydrogenase; (1 of 1) PF01756//PF02770 - Acyl-CoA oxidase (ACOX) // Acyl-CoA dehydrogenase, middle domain (Acyl-CoA_dh_M) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCATCTTGTGCCCCATGTCCTCCACACGCACGTTGGCGCACACGCTCA
Internal bar code:GTTGAGTGCTCTACCGATTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:320
LEAP-Seq percent confirming:42.8571
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAAGCCCAAAGAAGCCAA
Suggested primer 2:GGGGAGGAGATGGGTTTTGG