| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053688 |
| Chromosome: | chromosome 16 |
| Location: | 5202467 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684700 | (1 of 1) PF10309 - Protein of unknown function (DUF2414) (DUF2414) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGTCGGCAGGAGGTGATAAGGGACGCTAAGTGCCCGTGAGAGTCAGT |
| Internal bar code: | GATGGGGAATGTATATACTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4663 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGAATGCCGTTGCTGACA |
| Suggested primer 2: | GCCTAGTACTTCAGCGCGAT |