Insertion junction: CLIP2.053693_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.053693
Chromosome:chromosome 16
Location:6281593
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g676200 (1 of 3) PTHR10751:SF2 - GUANYLATE-BINDING PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCACATGCTGCCCCGCCTGCTGCCGCCCTGGCGCTGCAGGGCTGTGGA
Internal bar code:TAGCGGTTAACGCGTAGAATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:331
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCGGACTGTCCCTTCTTG
Suggested primer 2:TTCGAAGTGGCAAGCAGCTA