Insertion junction: CLIP2.053705_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.053705
Chromosome:chromosome 10
Location:4409382
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g450300 (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAGGTGGCTGACGCGGCGCTGAGGCATGGTGCCGTGGTGGCTGCTGCT
Internal bar code:GCGGAACTAAGAATTGTTCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:976
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTCCTCACATCCCACTG
Suggested primer 2:CCGCCCTCTTCTCCAACAAT