Insertion junction: CLIP2.053716_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.053716
Chromosome:chromosome 1
Location:7701340
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g054150 NTRC1,NTR4 (1 of 1) PTHR10438//PTHR22912//PTHR22912:SF48 - THIOREDOXIN // DISULFIDE OXIDOREDUCTASE // NADPH-DEPENDENT THIOREDOXIN REDUCTASE 3; NADPH-dependent thioredoxin reductase C, chloroplastic intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGGTTCGCGCGCGGGATTGCGGCGGCGGAGCAACAAGTCGCGCCCGCT
Internal bar code:AAGCAGCTTGGGTGTGCAAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3553
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:GAGATCCTGATGGTCGCCAG
Suggested primer 2:TACTTGGTCACGTACACGGC