Insertion junction: CLIP2.053721_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.053721
Chromosome:chromosome 1
Location:3740553
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g024400 TRS85 Component of TRAPP complex, TRS85-like; (1 of 1) PF12739 - ER-Golgi trafficking TRAPP I complex 85 kDa subunit (TRAPPC-Trs85) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAACAGATTACCACCCACCCGCTCGCCCACCTGGACACGCGTTTGAC
Internal bar code:CAATTCGTAACGTAAAACCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:6.66667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCCACCCATTTTTCTGTG
Suggested primer 2:GCAGGGACGATGGATATGGG