Insertion junction: CLIP2.053730_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.053730
Chromosome:chromosome 2
Location:3073646
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095900 ROC114,ROC108 Rhythm Of Chloroplast 114; (1 of 13) PF00646 - F-box domain (F-box) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACATGGCTTCGAAAGAAGTGGTTTCCGTGGTCGACGGTTCATCTAGGCA
Internal bar code:ATATACCCACAGTGCTGGCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:658
LEAP-Seq percent confirming:41.6667
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCAACCAGTTGCACAA
Suggested primer 2:AGTTCCCCCGCATCAAGAAG