| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053746 |
| Chromosome: | chromosome 13 |
| Location: | 4053840 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590800 | HTA21,HTA37 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGGCAAGAAGGCCGTGAGCCGCAGCGCCAAGGCGGGCCTGCAATTCC |
| Internal bar code: | GAAATGCAGGTTCACCGTTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4250 |
| LEAP-Seq percent confirming: | 54.5455 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGCAGGTTGGCACTACC |
| Suggested primer 2: | TCCTTGGCAGCCTTCTTAGC |