| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053757 |
| Chromosome: | chromosome 2 |
| Location: | 5986139 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118400 | ZCP2 | Zn chaperone; (1 of 5) PTHR13748:SF4 - COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCAGCCATGTATGGGGCACGCGCACCTGTCCGCCACCTCCTCGATGC |
| Internal bar code: | AGTGGATGGAGGTAGGACGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2818 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCACTGGCATTTCCGAG |
| Suggested primer 2: | CCGTTAACAATCCCCGGGAA |