| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053762 |
| Chromosome: | chromosome 7 |
| Location: | 6068026 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355300 | BES5 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGGCGGAACGCATCCCCCGGACGCCAAGCGACATAGCGCATGCATGG |
| Internal bar code: | TTCTGTTCGTGGCTGTTGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 57.1429 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTTGCAGGGATGGGAGG |
| Suggested primer 2: | GACCAACAGCAGCTACAGGT |