| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053776 |
| Chromosome: | chromosome 1 |
| Location: | 3376116 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021900 | PUS11 | (1 of 1) PTHR11142:SF10 - TRNA PSEUDOURIDINE SYNTHASE; RNA pseudouridine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGTCCGTTGCAGTGACGGCTGCAGCTCCTGCTTGCCCGCATGTAGA |
| Internal bar code: | GACAATGTGTGACGGCGAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1373 |
| LEAP-Seq percent confirming: | 13.3333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCTTAGCCCTACCGATG |
| Suggested primer 2: | CTAGCCGTCTGCCTGAACAA |