| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053777 |
| Chromosome: | chromosome 16 |
| Location: | 4056058 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690350 | SNR6 | SNARE-associated Golgi protein; (1 of 1) PTHR12677:SF24 - TRANSMEMBRANE PROTEIN 64 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAATGCTGCATCAGGTGTGAAAGGAGCGGCAGGCCGCGCGTCTGACCG |
| Internal bar code: | GGCCGCTTTCGGTCCCTACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2455 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTTTCGACGCGTTATCT |
| Suggested primer 2: | ACTGCCTGCATACACTTCCC |