| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053787 |
| Chromosome: | chromosome 12 |
| Location: | 9368329 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541700 | OPR54 | OctotricoPeptide Repeat protein 54 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAATGGAGGGGCGGGCCGGAGGAGTGGAGGGCGGGCGGCGCGGTGGG |
| Internal bar code: | TACAGAGCGGGCAGATATGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 6.66667 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGACATGCAGCGCTGTAA |
| Suggested primer 2: | CGTATGGATGCACAACAGCG |