Insertion junction: CLIP2.053788_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.053788
Chromosome:chromosome 2
Location:517238
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g076650 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGATCTTGCTCTTGCGAACATAGCACATAAGAAAATAGTGCTACAAGA
Internal bar code:TTTTACTTGGGGTCTTTCATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:965
LEAP-Seq percent confirming:30.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TCGAACACGCAGACTCGAAA
Suggested primer 2:CCGTGTCCGTGATCGATGAT