| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053819 |
| Chromosome: | chromosome 5 |
| Location: | 2877857 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| lncRNA_TCONS_00174559 |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCCACCCCGGGGCACATTCAGTGCGGCCGCAGCCACACAGGGGTAG |
| Internal bar code: | TGTGAAAGTCGAGTCGTACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2400 |
| LEAP-Seq percent confirming: | 48.0769 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTGGGTGGATTCCGCAT |
| Suggested primer 2: | AGTGGTTAGTATCCCCGCCT |