Insertion junction: CLIP2.053832_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.053832
Chromosome:chromosome 16
Location:4538634
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g671450 FAO12 FAD-dependent oxidoreductase; (1 of 1) PTHR13847//PTHR13847:SF150 - FAD NAD BINDING OXIDOREDUCTASES // OXIDOREDUCTASE TDA3-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCGCGCTGCTGGACGGCGCGGTGGCGGCGGGCGCGCGCCTGGTCACT
Internal bar code:ATGAGTTCCCGGGGACGCCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1532
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGGGAAACAGCTGGCAT
Suggested primer 2:AGAGGAATATGGTGCTGGCG