| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053838 |
| Chromosome: | chromosome 6 |
| Location: | 1136669 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257800 | POL1B,POL1-1 | (1 of 2) IPR001098//IPR002298 - DNA-directed DNA polymerase, family A, palm domain // DNA polymerase A; DNA polymerase I, probably mitochondrial | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGGGCACGACAGACATCATGCAGCCATAATGCAGTGTACTTGCGCAG |
| Internal bar code: | CAATGGATTACTACAGGTACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1571 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGGTATCGTATCCGGGG |
| Suggested primer 2: | ATCTCATCCAGCAGCGACAG |