Insertion junction: CLIP2.053847_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.053847
Chromosome:chromosome 1
Location:6501428
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g045903 DGAT1 (1 of 1) K11155 - diacylglycerol O-acyltransferase 1 (DGAT1); Diacylglycerol O-acyltransferase, Type 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATTCCATTGAGCTCGAGCACATCACCCACCACACCCACATACCCTCAT
Internal bar code:GGTAAGATACTCCAAAGGTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:85
LEAP-Seq percent confirming:2.43902
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCACCACGCATACTCACA
Suggested primer 2:CAGGGGGTGTAGGAGACAGA