| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.053851 |
| Chromosome: | chromosome 16 |
| Location: | 397890 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGAAACTCGGCCGCGTGACACACACCGGTCTGGTGGCTAATCCGAGT |
| Internal bar code: | GGCTATATTCGGTAGCAACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTGAATGCGCTTCGACG |
| Suggested primer 2: | TCGTGTACTGCGGATCTTCG |