Insertion junction: CLIP2.053852_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.053852
Chromosome:chromosome 12
Location:4220369
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g518050 FAP261 Flagellar Associated Protein 261; (1 of 2) PTHR16306:SF0 - TRANSLIN-ASSOCIATED FACTOR X-INTERACTING PROTEIN 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCATATCCACCACGCGCCGTGACGTGCTGGCCATCAGGTGCGCGGCC
Internal bar code:TAGGATATTCCTGGTTAGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1602
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGTGAAAGGAGGAAGGC
Suggested primer 2:GCTCTCGAACCCAATCCACA