| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053860 |
| Chromosome: | chromosome 11 |
| Location: | 1183966 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467683 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCTCAGTCGCTGGAGCGCTGGGTTTCGGCGTGCCTGTGGTTGCCTGC |
| Internal bar code: | CATAGCCGAATTTGATATCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2197 |
| LEAP-Seq percent confirming: | 65.2174 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCGTCAGTTTGGAAGGA |
| Suggested primer 2: | CCGTATGCATGCTTTGACGG |