Insertion junction: CLIP2.053862_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.053862
Chromosome:chromosome 6
Location:1403600
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g259700 (1 of 6) 3.1.2.2 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCAACCTCGCCCCGCTGCACGAGGCGCCGCACCATCACAGCCACAGC
Internal bar code:GGTATCATAGACTGTTGTAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1418
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGAGACCAAGCGAAACCA
Suggested primer 2:CTTTTGCTAGTGCGCTTCCC