Insertion junction: CLIP2.053867_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.053867
Chromosome:chromosome 11
Location:581513
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467607 (1 of 3) 2.7.11.17//2.7.11.25 - Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase // Mitogen-activated protein kinase kinase kinase / MLTK intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCTTTACCCGCCCACTTAGCCACCCACGCACCAGTTGCGCCAGACATA
Internal bar code:AAGTCTCCATTTGTCAGACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2234
LEAP-Seq percent confirming:36.3636
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TAACAAGGAGGAGCAGCTGC
Suggested primer 2:CACACAACGCCTGACTTTGG