Insertion junction: CLIP2.053874_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.053874
Chromosome:chromosome 3
Location:5769617
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g187500 (1 of 1) PTHR23339:SF64 - CYCLIN-DEPENDENT KINASE INHIBITOR 3; Dual specificity phosphatase outside_mRNA
Cre03.g187550 (1 of 1) PF04858 - TH1 protein (TH1) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAATGCTGGACAAGTCGATAGAAGTCGGTGTGGTGCGTTCTAGGAATCC
Internal bar code:TTCAACATATCTCTGGTGCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:666
LEAP-Seq percent confirming:24.1379
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTTAGACCACGGTGACA
Suggested primer 2:TCAAGTTGCGTGCATGTTGG