| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053881 |
| Chromosome: | chromosome 2 |
| Location: | 6043290 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118801 | GTR | Glycosyl transferase; (1 of 1) 2.4.1.246 - Mannosylfructose-phosphate synthase / MFPS | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGAAGCCACGTACCGAAACGGGCCATGCAAGCATCGGCGGCCAGCGG |
| Internal bar code: | TGGCGGCGATCTTATCATGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2337 |
| LEAP-Seq percent confirming: | 47.2727 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGTGAACTCTGTGAGGT |
| Suggested primer 2: | TGGCAGTGGCTACTCAAGTG |