Insertion junction: CLIP2.053887_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.053887
Chromosome:chromosome 12
Location:4605133
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g521851 (1 of 3) PF04982 - HPP family (HPP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCCCCCGACGTCCCCCTGCTCGCTGTGTCCCGCCTGGCGCCCACAGC
Internal bar code:CCATATAGTAAATTCGGTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:437
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTGTTGGTTTTGGTCCG
Suggested primer 2:AGGTGTGATCGGACACGTTC