Insertion junction: CLIP2.053891_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.053891
Chromosome:chromosome 7
Location:1527445
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g324400 VPS24 Subunit of the ESCRT-III complex; (1 of 1) K12193 - charged multivesicular body protein 3 (VPS24, CHMP3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCCCAACCCGCACTGACCGCGAATTTGCCTGTCCAAGCCACGTTGCT
Internal bar code:TCGGCCGTACGTTGTCTCGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:4528
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGCAGCATCATTCGTCCT
Suggested primer 2:GCGCCTTGATGATCGTGTTC