Insertion junction: CLIP2.053895_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.053895
Chromosome:chromosome 2
Location:4402193
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g105700 (1 of 1) PF09837 - Uncharacterized protein conserved in bacteria (DUF2064) (DUF2064) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTTCGCCCCCCCCCCCATGTCCGCCCTCGCCTCGAACAGCTGCTCCGC
Internal bar code:GGACTGTCCATCTTTGTTTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1861
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCAGGTCATCATCACCGG
Suggested primer 2:CATTTTCCCGGTTGCAAGCA