| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053896 |
| Chromosome: | chromosome 6 |
| Location: | 4689003 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278850 | MND1 | GAJ-like protein; (1 of 1) PF03962 - Mnd1 family (Mnd1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACGTTAGTCGGCCGTGACAGGGGAAGGCAACCACACGGTTTAGCGCA |
| Internal bar code: | GAAAGTATCCAGGGACTGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3783 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTACAAGTTCTGGGGCT |
| Suggested primer 2: | CGTCAGTTCTTACGCATGCG |