| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053897 |
| Chromosome: | chromosome 9 |
| Location: | 6320690 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414100 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTCCTGGATGCTGCCGTGGTAGCCGTCTCTCTGCTGGCTGAGCGTCA |
| Internal bar code: | GCAGTACTGTACTTAAATCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1385 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACTCCCAAATGAGCTGG |
| Suggested primer 2: | TGAACTCGTGTCCAGGAACG |