| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053897 |
| Chromosome: | chromosome 9 |
| Location: | 6320692 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414100 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGTCCGCGGCCGACACCCTCAGCGCTTTGCTGCTTACAAAGCGCCCCT |
| Internal bar code: | TGGGGTGGGTATCTTCGTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3773 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACTCGTGTCCAGGAACG |
| Suggested primer 2: | ACCACTCCCAAATGAGCTGG |