| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053912 |
| Chromosome: | chromosome 7 |
| Location: | 864197 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318551 | CYA8 | Adenylate cyclase; (1 of 1) PF00211//PF13855 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Leucine rich repeat (LRR_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACTCTCACAATGCAGGAACCTGGACCCCGAGTATTATCTGTACG |
| Internal bar code: | ACGTTAAGGGTCTTGGTCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1387 |
| LEAP-Seq percent confirming: | 5.88235 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATAGTCCACAGGCGCACG |
| Suggested primer 2: | TCGCACTGCTGACATGACTT |