| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.053914 |
| Chromosome: | chromosome 12 |
| Location: | 5336314 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCAGGCCCCTCTGCTTGCTGCTGCTGACTGCGCCGGAAGACAATTGC |
| Internal bar code: | ATTTTCAATACGCGTGCTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 11.1111 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCATCTTGCTTACCGCTT |
| Suggested primer 2: | CTAATGAGCGTGTGCAGCAC |